TGF-β1信号通路在副猪嗜血杆菌侵入3D4/21细胞中的作用

doi:10.11843/j.issn.0366-6964.2018.08.017

Abstract

Abstract:

This study aimed to investigate the relationship between the expression of transforming growth factor β1 (TGF-β1), leading to the expression of fibronectin (Fn)/α5 integrin, and the invasion of Haemophilus parasuis (H. parasuis) in porcine alveolar macrophage cell line 3D4/21. The optimal adherence/invasion conditions of H. parasuis to 3D4/21 cells were determined by a series of adherence/invasion experiments. TGF-β1 expression level in the cells infected by H. parasuis was detected using Q-PCR and ELISA. Treatment of cells with recombinant TGF-β1 prior to H. parasuis infection, CFU and flow cytometry were used to confirm the relationship between bacteria variation and the expression of TGF-β1. siRNA interference was used to down regulate the expression of Fn/α5 to confirm the relationship between the expression of Fn/α5 integrin and bacteria invasion. The H. parasuis infection resulted in the elevation of TGF-β1 expression in cells. Treatment of 3D4/21 cells with TGF-β1 prior to infection significantly increased the invasion of H. parasuis. Flow cytometry revealed that the expression of Fn/α5 integrin was increased in 3D4/21 cells treated with recombinant TGF-β1 and H. parasuis. Small interfering RNAs (siRNA) were used to down regulate the expression of Fn and α5 integrin in 3D4/21 cells, which significantly decreased the invasion of H. parasuis. However, H. parasuis attachment was decreased only on cells treated with the α5 integrin siRNA, there was no corresponding decrease in attachment to cells treated with Fn siRNA. These results demonstrate that expression of TGF-β1 and α5 integrin promote the invasion of H. parasuis to 3D4/21 cells and play an important role during this process.

CLC Number:

S852.613

SHEN Yi-juan, ZHOU Ni-ni, ZHANG Jian-song, WANG Mei-fen, LI Yu-feng, JIANG Ping. TGF-β1 Signaling Stimulates Invasion of Haemophilus parasuis into 3D4/21 Cells[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(8): 1720-1726.

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TGF-β1 Signaling Stimulates Invasion of Haemophilus parasuis into 3D4/21 Cells

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